International Journal of Science , Engineering and Management (IJSEM)

Monthly Journal for Science , Engineering and Management

Volume2,August 2017,

Topic : Gentipicroside Elicitation in Shoot Cultures of Gentiana kurroo and Swertia chirayita with Molecular Authentication

Authors:Payal Kotvi || Rhythem Sharma,Satyam Singh, Hemant Sood

Abstract:Gentiana kurroo and Swertia chirayita are critically endangered medicinal herbs which belong to the family Gentianance . They are widely distributed in areas of Jammu and Kashmir, Himachal Pradesh and Uttrakhand and grow between altitude ranges of 1500-3500 m. These two plants have served to be an important source of secondary metabolites i.e gentiopicroside, swertamarin ,amarogentin and maniferin which have high medicinal properties. In the present study an attempt has been made to elicit these phytochemicals by using varied concentration of elicitors like Methyl jasmonate (50-100 μM), Sodium nitroprusside (25-75 μM), Salicylic acid (25-50 μM) and Seaweed (1-3%) in Murashige and Skoog (MS) medium. These phytochemicals were quantified by HPLC and were also observed for their growth and development. The best results were acquired on Seaweed (2%) which gave 0.34% of gentiopicroside in Gentiana kurroo at an interval of 15 days. The other two best results were observed in Salicylic acid (50 μM) i.e 0.20% and Sodium nitropruside (50 μM) i.e 0.19% in Gentiana kurroo at an interval of 30 days. Elicited plants of Swertia chirayita have shown a lower concentration of gentiopicroside i.e 0.1%. The HPLC analysis of shoot cultures grown in MS supplemented with Sodium nitroprusside, Salicylic acid and seaweed elicited cultures revealed enhanced concentration of swertiamarin, amarogentin and manigiferin in Swertia chirayita. This study is the first to utilize Swertia chirayita as a source of gentiopicroside. Since these two plants have been exploited for their metabolites and very few efforts have been made to cultivate them. This study can serve a mean to acquire an enhanced amount of these metabolites to meet the demands of the pharmaceutical sectors. We have also checked the amplification of four genes from conserved pathways (MVA & non- MEP) encoding HMGR, PMK , ISPD and G10H just to authenticate the contribution of key genes for the medicinal compounds biosynthesis so that those could be used for genetic modification for improving the medicinal compounds production..

Keywords: Gentiana kurroo, Swertia chirayita, Elicitation, Gentiopicroside and HPLC.

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DOI: 01.1617/vol4/iss8/pid26459

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Dr.Muhammad Ishtiaq,
Mirpur University of
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Mirpur, Azad Kasmir.

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ISSN(Online): 2456-1304

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Thomson Reuters ID : q-6288-2016.
ORCiD Research ID : 0000-0001-9540-6799

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Journal Code : IJSEM
Electronic ISSN : 2456-1304
Impact Factor : 2.7
Frequency : Monthly
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