International Journal of Science , Engineering and Management (IJSEM)

Monthly Journal for Science , Engineering and Management

Volume2,September 2017,

Topic : Toll-like receptors and calcium-activated chloride/potassium channels as immunomodulators of allergic airway inflammation and asthma: a public health research experience in State of Nebraska, USA

Authors:Saumya Pandey ||

Abstract:Allergic airway inflammation and asthma have emerged as major public health problem in the Midwest, United States of America. Targeting Toll-like receptor (TLR) signaling and Calcium-activated Chloride (ClCa)/Potassium (KCa) Channels in unraveling the cellular/molecular mechanisms underlying genetic susceptibility to inflammatory diseases in specific human patient population subset(s) is emerging as an attractive immunotherapeutic pharmacological strategy in management/prevention of asthma. Objectives: My pilot study aimed to investigate the role of TLRs and ClCa/KCa ion channels in human bronchial epithelial cells (NHBEC, BEAS-2B from ATCC), and eosinophils-derived from asthma patients of North American ethnicity. Methods: Whole cell patch clamp electrophysiological recordings were conducted for ClCa and KCa currents in cultured cells (passages P3-P7) grown on cover-slips. RNA and Protein were extracted from NHBECs, BEAS-2B and eosinophils using Trizol and RIPA methods. Pipette and bath solutions for electrophysiology were subjected to stringent pH and osmolality checks prior.

Keywords: Asthma, Biomarkers, Calcium activated Chloride/Potassium Channels, Toll-like receptors

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DOI: 01.1617/vol4/iss9/pid25743

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Dr.Muhammad Ishtiaq,
Mirpur University of
Science & Technology,
Mirpur, Azad Kasmir.

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ISSN(Online): 2456-1304

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Thomson Reuters ID : q-6288-2016.
ORCiD Research ID : 0000-0001-9540-6799

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Journal Code : IJSEM
Electronic ISSN : 2456-1304
Impact Factor : 2.7
Frequency : Monthly
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