International Journal of Science , Engineering and Management (IJSEM)

Monthly Journal for Science , Engineering and Management

Volume2,September 2017,

Topic : Detection of Toxin Genes in E. Coliisolate PE74 by PCR analysis

Authors:Mary Conice || Usha M.S.

Abstract:E.coliisolatepe74 culture which showed positive for cytotoxic effect was selected for PCR analysis. On genomic DNA isolation, isolate pe74 showed prominent band on agarose gel electrophoresis. Isolatepe74 was analyzed for the presence of stx, stx2f, stx2, stx2c, stx2e, eaeA, stx2dandhlyA using different primers. On PCR amplification isolate pe74 showed no amplified product for Stx1 however only primer dimmers were observed in sample. PCR analysis of isolate pe74 showed positive to stx2f with a band close to 150 bp and a band between 100 and 200 bp for stx2c..

Keywords: E. coli, PCR analysis, toxin production

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DOI: 01.1617/vol4/iss9/pid49316


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Editor-in-Chief

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Dr.Muhammad Ishtiaq,
Mirpur University of
Science & Technology,
Mirpur, Azad Kasmir.

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IMPACT FACTOR: 2.7

ISSN(Online): 2456-1304

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Thomson Reuters ID : q-6288-2016.
ORCiD Research ID : 0000-0001-9540-6799

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Journal Code : IJSEM
Electronic ISSN : 2456-1304
Impact Factor : 2.7
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