International Journal of Science , Engineering and Management (IJSEM)

Monthly Journal for Science , Engineering and Management

Volume2,December 2017,

Topic : Quantitative Analysis of Methyl-Coenzyme M Reductase (MCRA) Gene in a Biogas Producing Reactor Treating Brewery Wastewater

Authors:Abimbola M. Enitan || John O. Odiyo, Feroz M. Swalaha

Abstract:Among the organisms that are involved in digestion of organic matter degradation methanogens are the major microbial group responsible for methane production. This study quantify the concentration of total bacteria and methyl-coenzyme M reductase α-subunit (mcrA) gene, specific functional gene for methane-producing Archaeal in an anaerobic reactor treating brewery wastewater using quantitative real-time polymerase chain reaction (Q-PCR). Primer sets targeting mcrA gene and total bacteria were used to detect and quantify the concentration present in the sludge samples. Q-PCR results showed that a high amount of mcrA gene that codes for the functional enzyme in methane producing Archaea are present in the reactor. However, the ratio of Archaea to bacteria concentration is lower and this revealed that the quantity of methane producing communities needs to be enhanced in this reactor, in order to increase biofuel production. The results further increased our understanding on the ability of methanogens to grow in high concentration at an optimum reactor performance in anaerobic condition to transform organic substrate present in industrial wastes into biogas as source of renewable energy..

Keywords: Archaea, brewery wastewater, methanogens, quantitative PCR.

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DOI: 01.1617/vol4/iss12/pid60473

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Editor Image

Dr.Muhammad Ishtiaq,
Mirpur University of
Science & Technology,
Mirpur, Azad Kasmir.

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ISSN(Online): 2456-1304

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Thomson Reuters ID : q-6288-2016.
ORCiD Research ID : 0000-0001-9540-6799

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Journal Code : IJSEM
Electronic ISSN : 2456-1304
Impact Factor : 2.7
Frequency : Monthly
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