International Journal of Science , Engineering and Management (IJSEM)

Monthly Journal for Science , Engineering and Management

Volume3,May 2018,

Topic : To study the effects of Homothorax during Drosophila Central Nervous system development

Authors:Sreya Mukhopadhyay || Venu Gopal Reddy

Abstract:Hippo signalling has emerged as a novel tumor suppressor pathway that plays an important role in the regulation of tissue and organ size during development. The Hippo pathway consists of a core kinase cascade in which Hpo phosphorylates the protein kinase Warts. Activated Wts then go and phosphorylate and inactivate the transcriptional coactivator Yorkie (Yki). Wts promotes the association of Yki with 14-3-3 proteins, which helps anchor Yki in the cytoplasm and prevent its transport to the nucleus. Homothorax -a homeodomain transcription factor- in conjunction with Yki has been shown to regulate micro RNA bantam, a positive regulator of growth. Works from various laboratories have shown that down regulation of Homothorax reduced epithelial cell growth in eye discs. Here, we tried to characterize the effects of Homothorax knockdown in glial cells and its effects on CNS growth and development at various stages of Drosophila development. Previous work from our laboratory showed that glia specific knockdown of Homothorax does not reduce the glial cell number. However, in the present work, we notice that the mutant larvae displayed wedge shaped CNS phenotype with extended optic lobes. Further, we also studied the effect of Hth RNAi in embryos. In addition to that, we also noticed that Hth RNAi mutant pupae never enclosed into adults. Further, both to confirm the specificity of RNAi and rule out the off target effects of RNAi, we performed rescue experiments using mammalian homologue of Homothorax i.e. UAS Myc Meis. Glia specific expression of UAS Myc Meis completely rescued the Hth RNAi phenotype to normal. This experiment allowed us to study the functional conservation of Homothorax. Further it would be very interesting to study and characterize these phenotypes at both cellular and molecular level..

Keywords: Hippo signalling, Homothorax, Drosophila, Central nervous system.

Download Paper

DOI: 01.1617/vol5/iss5/pid74912

Related Articles

Solidification Pattern in V-Groove Submerged Arc Welded Plate

Authors: Anirudhha Ghosh ||

Doi : 01.1617/vol1iss2pid0010005

Volume1 ,June 2018.

In Air Synthesis of Psyllium Based OrganoInorganic Hybrid Ion Exchanger, its Characterization and Studies

Authors: Rajeev Jindal || Rachna Sharma ,Mithu Maiti, Harpreet Kaur

Doi : 01.1617/vol1iss2pid0010006

Volume1 ,June 2018.

A Study on Utilization of Biomass Waste Pellets as an Alternate Fuel

Authors: Simran S. N || Shivani S. B, Raji S,D. D. Sarode

Doi : 01.1617/vol1iss2pid0010024

Volume1 ,August 2018.

Study on Deforestation and Applicable Related Act in India

Authors: Dr. S. Kanthimathinathan ||

Doi : 01.1617/vol1iss2pid0010030

Volume1 ,August 2018.

Store Atmospherics: The Key to Retail Shoppability

Authors: Dr.Smitha Nayak || Dr.K.V.M.Varambally

Doi : 01.1617/vol1iss2pid0010042

Volume1 ,October 2018.

Study of Different Pragmatic Approaches and Testing Strategies for Stand Alone/ Desktop Application

Authors: Prof. Vaishali Jawale || Dr. K. Nirmala Kumaraswamy

Doi : 01.1617/vol1iss2pid0010046

Volume1 ,October 2018.

A Survey on Secured ECG Monitoring System Using Wireless Sensor Networks and IOT

Authors: Femi Antony || ANN Mary Joseph,Jency K Davis, Diffeena Stenislawoos

Doi : 01.1617/vol1iss2pid0010050

Volume1 ,October 2018.

Reliable Human Identification using IRIS as a Biometric

Authors: Jayshree Sali || C.B.Kadu

Doi : 01.1617/vol1iss2pid0010057

Volume1 ,December 2018.

Development of Analytical Framework on Optimization of Construction Equipment for Multiple Infrastructure Projects

Authors: Mr. Amit Shriwas || Dr. Sudhir Nigam

Doi : 01.1617/vol1iss2pid0010066

Volume1 ,December 2018.

Online Big File Storage Mechanism

Authors: Ms.Noopur Adarsh Purwar || Prof.Nilima Nikam,Prof.Vaishali Londhe

Doi : 01.1617/vol1iss2pid0010067

Volume1 ,December 2018.


Editor Image

Dr.Muhammad Ishtiaq,
Mirpur University of
Science & Technology,
Mirpur, Azad Kasmir.

View more


ISSN(Online): 2456-1304

Google Scholar Profile

Thomson Reuters ID : q-6288-2016.
ORCiD Research ID : 0000-0001-9540-6799

All Issues


Copy-right Form Paper Template


Journal Code : IJSEM
Electronic ISSN : 2456-1304
Impact Factor : 2.7
Frequency : Monthly
Contact :

IFERP Other Journals



Social Media