Systems biology is the computational and mathematical modeling of complex biological systems. It is a biology-based interdisciplinary field of study that focuses on complex interactions within biological systems, using a holistic approach to biological research. Genome scale metabolic flux analysis is an emerging in the field of system biology. Metabolic flux analysis (FBA) is an experimental fluxomics technique used to examine production and consumption rates of metabolites in a biological system. FBA can give a large number of mathematically acceptable solutions to the steady-state. However solutions of biological interest are the ones which produce the desired metabolites in the correct proportion of these metabolites. For instance when modeling the growth of an organism the objective function is generally defined as biomass. Performing various experiments in an invivo system is a difficult task and an expensive and time consuming process. Through system biology it is possible to bring the difficult an easy process. By constructing the stoichiometric metabolic equation of an organism and simulation through software’s make it easy to find the best possible solution for the higher production. This also helps in finding the better solution for knocking in and knocking out stratergies.