International Journal of Engineering Research in Electronics and Communication Engineering

Fractional Characterization of Bacteriocin-Like Material of Pseudomonas aeruginosa AND Determination of Its Antibacterial Activity Against b. Cereus and s. typhimurium

Author : Jayant Pawar ¹ Rabinder Henry ² Amit Patwardhan ³ Prakash V ⁴ Ashish Kumar Patel ⁵ E A Singh ⁶

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Date of Publication :22nd December 2017

Abstract: This study presents the fractional characterization of bacteriocin-like material (Inhibiting molecule showing properties of bacteriocin) produced by Bacteriocin-like material was found to be active against Bacillus cereus and Salmonella typhimurium.. The maximum antibacterial activity of Pseudomonas aeruginosa was recorded at 16th hour incubation in nutrient broth at 370C. The antibacterial activity was found to be extracellular since, cell-free supernatant showed inhibition against test organism. The inhibitor was found to have proteinaceous nature and same has been confirmed by inactivation of inhibitor after addition of proteolytic enzymes (trypsin and proteinase K) and 0.2N NaOH for alkaline hydrolysis. After prolonged refrigeration and incubation at high temperature (600C, 1200C and under autoclave) for 15 minutes, inhibitor was found to be active against Bacillus cereus and Salmonella typhimurium. Therefore, inhibitor produced by the Pseudomonas aeruginosa can be bacteriocinlikematerial and may find application in medicine and in minimally processed food stuffs

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