Date of Publication :8th June 2024

Abstract: Candida species are commensals of the gastrointestinal tract, mucous membranes, and skin. These are the endogenous opportunists that lead to the infection cycle. The consumption of immunosuppressants, indwelling devices, and broad-spectrum antibiotics has risen over the past few decades, leading to a noteworthy rise in the prevalence of Candida infections. The purpose of this study is to use conventional methods to speciate Candida. Methods: The study included 54 isolates of Candida from various clinical samples. Gram stain, germ tube testing, along with inoculation on readily accessible CHROM agar (HiMedia India) were performed on these isolates. The Dalmau technique was used to detect the formation of chlamydospores. Results: The study comprised 54 Candida isolates obtained from various clinical samples. Most of the Candida isolates in the present investigation came from urine (50%) and were followed by sputum (26%), high vaginal swabs (14%), pus from surgical sites, and other sources (10%). The most prevalent species of candida was found to be Candida albicans 28/54 (51.85%), followed by C. tropicalis 08/54(14.81%), C. krusei 07/54(12.9%), C. dubliniensis 06/54(11.11%) and C. glabrata 5/54(9.25%). Conclusion: In order to treat the disease, it is essential to quickly and accurately identify the species of Candida that are causing it, that is, to recognize any species that may naturally defy conventional antifungal drugs. Non-albicans candida species, like C. tropicalis, C. dubliniensis, C. krusei, as well as C. glabrata, are also being removed from different clinical specimens more frequently than Candida albicans. For the quick and affordable identification of candida species, use CHROM agar.

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