Author : Dr. Atreyi Ghosh 1
Date of Publication :7th April 2021
Abstract: Bacterial strain capable of producing extracellular blue-green pigment was isolated from soil samples. The blue-green extracellular supernatant showed antimicrobial effect against F. oxysporum and E. coli DH5α on solid culture. Minimum inhibitory concentration (MIC) for both the microorganisms were measured by the Kirby-Bauer method. The extracellular supernatant had shown more inhibitory effect on E. coli DH5α than F. oxysporum confirming better antibacterial efficiency than antifungal one. The characteristic blue colour of the chloroform extract obtained from the extracellular matrix of the bacterial culture indicates the pigment and the bacterial isolate to be pyocyanin and Pseudomonas aeruginosa respectively.
Reference :
-
- Abou Raji El Feghali P. and Nawas T , Extraction and purification of pyocyanin: a simpler and more reliable method Volume 4 Issue 6 – 2018
- Anjaiah V, Koedam N, Nowak-Thompson B, Loper J E, Höfte M, Tambong J T and Cornelis P, Involvement of Phenazines and Anthranilate in the Antagonism of Pseudomonas aeruginosa PNA1 and Tn5 Derivatives Toward Fusarium spp. and Pythium spp. Vol. 11, No. 9, 1998, pp. 847–854. Publication no. M-1998-0615-02
- El-Fouly M.Z., Sharaf A.M., Shahin A.A.M., El-Bialy Heba A., Omara A.M.A., Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa, Journal of Radiation research and Applied Science, Volume 8, 2015 - Issue 1
- Islam M D, Nain Z, Alam M K, Banu N A and Islam M R, In vitro study of biocontrol potential of rhizospheric Pseudomonas aeruginosa against Fusarium oxysporum f. sp. Cucumerinum, Egyptian Journal of Biological Pest Control (2018) 28:90
- Jayaseelan S, Ramaswamy D, Dharmaraj S, Pyocyanin: production, applications, challenges and new insights, World J Microbiol Biotechnol, DOI 10.1007/s11274-013- 1552-5
- Marrez D A and Mohamad H S, Biological activity and applications of pyocyanin produced by Pseudomonas aeruginosa, Open Access Journal Biomedical Science. January 2020 - 1(4): 140-144
- Osama M Darwesh, Khouloud M Barakat, Magdy Z Mattar , Shawky Z Sabae , Sahar H Hassan, Biointerface Research in Applied Chemistry, Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa, Volume 9, Issue 5, 2019, 4334 - 4339 ISSN 2069-5837
- Stephen S. Baron, John J. Rowe, Antibiotic Action of Pyocyanin, Antimicrobial Agents and Chemotherapy, Dec. 1981, p. 814-820.