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ISSN : 2394-6849 (Online)

International Journal of Engineering Research in Electronics and Communication Engineering(IJERECE)

Monthly Journal for Electronics and Communication Engineering

Open Access Journal

International Journal of Science Engineering and Management (IJSEM)

Monthly Journal for Science Engineering and Management

ISSN : 2456-1304 (Online)

DNA Barcoding of Cynadon Dactylon and Molecular Characterization of Its Endophytic Fungi

Author : Dhananjaiah T M 1 Shalmali Chatterjee 2 Chandrika S Tanthry 3 Shruthi SD 4

Date of Publication :15th July 2020

Abstract: Medicinal plant-like Cynodon dactylon has been used for millenary years in folk medicines and still have considerable value in the area of ethnomedicine. Prior to the study of the medicinal importance of the sample, identification of the plant sample as Cynodon dactylon was essential, which was done using the DNA barcoding technique, with the primer rbcl. DNA barcoding is the process of identification of species based on nucleotide diversity of short DNA segments. Investigation of this medicinal Durva plant shows that it harbors a wide variety of endophytes. Endophytes are widely distributed in nature and portray a balanced symbiotic continuum ranging from mutualism to parasitism in the course of a long period of co-evolution with contrasting kinds of ecological functions. Endophytic fungi from plants are exceptionally substantial due to the presence of certain novel bioactive compounds that bears promising application in the fields of agriculture, medicine and food industry. The identification of endophytic fungi present in the plant Cynodon dactylon was performed using the DNA barcoding technique. The paper also expounds on screening of the different kinds of extracellular enzymes secreted by the endophytic fungi isolated and identified.

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