Author : R. Latha 1
Date of Publication :31st July 2021
Abstract: Increasing need for the strategies to encounter the agrowaste management is a current challenge, as there is more number of agro based cottage industry blooming to meet out the need of the rural economy. Silkworm excrement from the Lepidopteran larvae Bombyx mori is one of the organic biowastes. As novel study silkworm excreta was used to synthesize silver and gold nanoparticles and explored the efficiency of this organic waste in the nanotechnology. Synthesized silver nanoparticles showed  maximum absorbance at 418 nm and gold nanoparticles at 555 nm scale. FTIR showed peaks at 34564.49cm -1 &1626.93cm -1 for silver nanoparticles and 3468.85cm -1 & 1636.04cm -1 for gold nanoparticles. Scanning electron microscopy revealed the average size of biosynthesized silver and gold nanoparticles were about 42 nm& 45 nm respectively. EDAX value of biosynthesised silver and gold nanoparticles showed 33.08% and 14.77% respectively. XRD show diffraction pattern of about 5 peaks for both silver and gold nanoparticles. Antimicrobial activity was assessed using the resazurin assay method. MIC values obtained for the tested organisms revealed antimicrobial activities.K. pneumoniae, showed MIC at 62.5 µg for silver nanoparticles and A. niger, A.flavus and C.albicans showed MIC at 125 µg for gold nanoparticles. IC 50 values of α- amylase activity was found to be 574 and 1075µg/ml for the silver and gold nanoparticles, respectively. IC 50 values are about 3521 and 415.97 µg/ml for the silver and gold nanoparticles, respectively, in α-glucosidase activity. Conclusively silver and gold nanoparticles synthesized from the silkworm excreta revealed antibacterial, antifungal and antidiabetic activities
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