Author : Dipeshkumar Patel 1
Date of Publication :31st July 2021
Abstract: Bacteriocins are small ribosomally synthesized antimicrobial peptides, that is produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for wide range of pH. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30 minutes. The well diffusion assay showed the bacteriocin produced by LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis presenting a wide spectrum of inhibition. The molecular weight of bacteriocin is 6 kDa and it was determined by SDS-PAGE. This bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. There is many bacteriocins have been investigated with respect to their potential use in promoting human, plant, and animal health, and also, as food biopreservatives. Bacteriocins produced by LAB are particularly interesting since several LAB have been granted as GRAS (Generally Recognized as Safe) status from FDA. Because it is not always possible to extract active bacteriocins secreted from cellsgrown in liquid medium, we developed a simple and inexpensive extraction procedure, using ammonium sulphate precipitation and DEAE Sephadex A-25 Column. By using Folin’s Lowery method, the concentration can be finding out. I hereby present a detailed procedure that leads to the rapid extraction of secreted bioactive bacteriocin peptides from the Lactic acid bacteria, isolated from curd sample. Also present a simple method for the optimization of bacteriocin for their detection of activity from the purified bacteriocin sample.
Reference :
-
- S. Akhtar, M. R. Sarkar, and A. Hossain, “Microbiological food safety; a dllemma of developing societies,” Critical reviews in microbiology, vol, 10. No. 4, pp. 348-359, 2013.
- Z. Muhammad, R. Ramzan, A. Abdelazez et al., “Assessment of the antimicrobial potentiality and functionality of L. plantarum strains isolated from the conventional inner Mongolian fermented cheese against foodborne pathogens,” Pathogens, vol. 8, no. 2, p. 71, 2019.
- L. G. Stoyanova, E. A. Ustyugova, and A. I. Netrusoy, “Antibacterial metabolites of lactic acid bacteria: their diversity and properties,” Applied Biochemistry and Microbiology, vol. 48, no. 3, pp. 229-243, 2012.
- S.C. Yang, C.H. Lin, C.T. Sung, and J. Y. Fang, “Antibacterial activities of bacteriocins: application in food and pharmaceuticals,” Frontiers in Microbiology, vol. 5, p. 241, 2014.
- Ennahar S., Sashihara T., Sonomoto K. and Ishizaki A. s.l. : FEMS Microbiol. Rev., Vol. 24, pp. 85-106. 2000.
- Pisabarro., Oscáriz J.C. and A.G. s.l. : Int.Microbiol., Vols. 4, pp.13-19, 2001.
- M. Nishie, J.-1. Nagao, and K. Sonomoto. Antibacterial peptides "bacteriocins": an overview of their diverse characteristics and applications. s.l.: Biocontrol science, Vol. 1 no. 1, pp. 1-16,, 2012.
- S. C. Yang, C. M. Lin, C.T. Sung, and J.Y. Fang. Antimicrobial activities of bacteriocin: application in food and pharmaceuticals.. s.l.: Frontiers in Microbiology, Vol. 5. 2014.
- A.nigam, D. Gupta, and A, Sharma. Treatment of infectious disease: beyong antibiotics. s.l.: Microbiological Research, Vols. 169, No. 9-10. pp. 643-651,2014.
- H., Roy P. s.l.: Med. Sci., Vols. 13, pp, 927-933, 1997.