Author : Sheetal Chanyal 1
Date of Publication :20th June 2016
Abstract: Fungal laccases are unit of multicopper oxidases (MCOs) with high biotechnological potential due to their capability to oxidize a wide range of aromatic contaminants using oxygen from the air. Albeit the numerous laccase like genes described in ascomycetous fungus fungi, ascomycete laccases are less completely studied than white rot fungus laccase.0’; We have a tendency to aim here to identify the laccase type Oxidoreductases which may be concerned within the decolourization of textile dyes by using laccase producing endophytic fungi and to characterize them as potential biotechnological tools. Fungal Laccase (benzenediol: O enzyme, EC 1.10.3.2) along with ferroxidases (EC one.16.3.1) and ascorbate oxidase (EC one.10.3.3) kind the family of multicopper oxidases (MCOs), which play major role in bioremediation of these toxic dyes. In view of application of Laccase in bioremediation producing, a total of six endophytic fungi were recovered from leaves of healthy plant of Cupresustorulosa employing standard isolation methods. These endophytes were morphologically characterized by using colony appearance on PDA and Lacto phenol cotton blue staining techniques aswell as 18SrDNA sequencing methods. Endophytic fungi were screened for Laccase assay as it plays a crucial role in detoxification of toxic dye for human health. Laccase assay were performed using substrate such as Guaiacol, nephthol and ABTS, Syringeldehyd, Tannic acid. Out of six endophytic fungi only one endophytic fungus showed strong Laccase activity using Guaicol and ABTS, syringeldehyd as substrate. These results suggest that laccase producing Daldinia sp. was effective in decolorizing of synthetic dyes and promising in bioremediation of wastewater in textile, food and aquaculture industries.
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