International Journal of Engineering Research in Electronics and Communication Engineering

Development of Disposable Activated Bombyx Mori Silk Fabric Biostrips for Pasteurization Test of Milk

Author : Kirti Rani ¹

Date of Publication :21st December 2016

Abstract: Pasteurization of Milk and milk products are essential because non-pasteurized milk has alkaline phosphatases (ALP) which cause intra-abdominal bacterial infections after drinking. Therefore, milk industries test the milk after pasteurization using various chemical methods including boiling. Here, objective of this work is to detect the presence of alkaline phosphatase in the pasteurized milk by using disposable dry-reagent biostips of sodium nitrate treated/ sodium chloride treated activated Bombyx mori silk fabric which are prepared by immobilization of bromocresol green and p-nitophenol phosphate which gave the instant color change from light blue to green if sample is not pasteurized and test is called positive (ALP +ve). This test was observed to carry out any ambient temperature conditions and prepared activated silk strips can be stored up to 7-8 months in non-humid conditions which might be considered cost effective and rapid biosensor employed for the qualitative estimation of alkaline phoshatase in the milk samples or milk products

Reference :

1. Saha, S and Ara, A., “Chemical and Microbiological Evaluation of Pasteurized Milk Available in Sylhet City of Bangladesh,” The Agriculturists, Vol 10(2), pp 104- 108, 2012.
2. Biqiang Chena, b., Chunhua Yinc, Yuanyuan Chenga, Weina Lia, Zhu-an Caob and Tianwei Tana., “Using silk woven fabric as support for lipase immobilization: The effect of surface hydrophilicity/hydrophobicity on enzymatic activity and stability”, Biomass and Bioenergy, 39, 59-66, 2012.
3. Rani, K., “Immobilzation of azadirachta indica alkaline phosphatase onto polyethylenimine-treated chlorinated woven Bombyx mori silk fabric”, International Journal of Biochemistry and Biotechnology Vol 1(1), pp 1-4, 2012.
4. Tulsani, N. B., Vikram, H., Kumar, H., Suman, Chaudhary, R., Rani, K., & Kumar, A., “Arsenazo III test strip for rapid detection of hardness of water”, Indian Journal Chemical Technology,Vol 8, pp 252–254, 2001.
5. Kumar, A., Kumar, H., Tulsani, N. B., & Joshi, A. P., “A quick, simple and reliable method for detection of urea in adulterated milk”, Oriental Journal of Chemistry,Vol 14, pp 189–198, 1998
6. Kumar, A., & Tulsani, N. B., “Dry-reagent test strips for diagnosis of diabetes”, Popular Science, Vol 5, pp 30– 33, 1997