International Journal of Engineering Research in Electronics and Communication Engineering

Isolation, Screening and Optimization of Microbial Cellulase

Author : Rahul G Badgha ¹ Ruby E Jalgaonwala ²

Date of Publication :20th July 2017

Abstract: Cellulase enzymes are biological molecules that act as a catalyst and help complex reaction to occur. Now day significant attentions have been devoted to the current knowledge of cellulase production and challenges in cellulase research especially in the direction of improving the process economics of various industries.Having potential application in various industries including pulp and paper, textile, food and laundry industries. Enzyme cellulase cause hydrolysis of the individual cellulose fibers to break it into smaller sugars units and finally producing glucose molecules. The cellulase producing bacteria were isolated from paper industry waste. A total of 9 isolates were obtained by the qualitative screening technique. Isolates coded PP-1 and PP-2 were efficient cellulase producers with specific enzyme activity of 181mU/ml and 189mU/ml respectively.Organism coded PP-2 gives maximum enzyme activity when characterized for pH, temperature, carbon and nitrogen sources.As pH 7.0,temperature 37ËšC, fructose andammonium phosphate as best carbon and nitrogen source. PP-2 counts to be best cellulase producer among the rest 9 isolates, having optimum growth condition between pH 5-7 and temperature 30ËšC â€“ 40ËšC. Potent isolate was characterized on the basis of morphological, structural, biochemical test and partially identified as Bacillus Sp. with the help of Bergeyâ€™sManual of systematic bacteriology.

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