International Journal of Engineering Research in Electronics and Communication Engineering

Gentipicroside Elicitation in Shoot Cultures of Gentiana kurroo and Swertia chirayita with Molecular Authentication

Author : Payal Kotvi ¹ Rhythem Sharma ² Satyam Singh ³ Hemant Sood ⁴

Date of Publication :23rd August 2017

Abstract: Gentiana kurroo and Swertia chirayita are critically endangered medicinal herbs which belong to the family Gentianance . They are widely distributed in areas of Jammu and Kashmir, Himachal Pradesh and Uttrakhand and grow between altitude ranges of 1500-3500 m. These two plants have served to be an important source of secondary metabolites i.e gentiopicroside, swertamarin ,amarogentin and maniferin which have high medicinal properties. In the present study an attempt has been made to elicit these phytochemicals by using varied concentration of elicitors like Methyl jasmonate (50-100 Î¼M), Sodium nitroprusside (25-75 Î¼M), Salicylic acid (25-50 Î¼M) and Seaweed (1-3%) in Murashige and Skoog (MS) medium. These phytochemicals were quantified by HPLC and were also observed for their growth and development. The best results were acquired on Seaweed (2%) which gave 0.34% of gentiopicroside in Gentiana kurroo at an interval of 15 days. The other two best results were observed in Salicylic acid (50 Î¼M) i.e 0.20% and Sodium nitropruside (50 Î¼M) i.e 0.19% in Gentiana kurroo at an interval of 30 days. Elicited plants of Swertia chirayita have shown a lower concentration of gentiopicroside i.e 0.1%. The HPLC analysis of shoot cultures grown in MS supplemented with Sodium nitroprusside, Salicylic acid and seaweed elicited cultures revealed enhanced concentration of swertiamarin, amarogentin and manigiferin in Swertia chirayita. This study is the first to utilize Swertia chirayita as a source of gentiopicroside. Since these two plants have been exploited for their metabolites and very few efforts have been made to cultivate them. This study can serve a mean to acquire an enhanced amount of these metabolites to meet the demands of the pharmaceutical sectors. We have also checked the amplification of four genes from conserved pathways (MVA & non- MEP) encoding HMGR, PMK , ISPD and G10H just to authenticate the contribution of key genes for the medicinal compounds biosynthesis so that those could be used for genetic modification for improving the medicinal compounds production.

Reference :

1. Y. Shimizu, K. Maeda, M. Kato and K. Shimomura, "Co-expression of GbMYB1 and GbMYC1 induces anthocyanin accumulation in roots of cultured Gynura bicolor DC. plantlet on methyl jasmonate treatment", Plant Physiology and Biochemistry, vol. 49, no. 2, pp. 159-167, 2011.
2. Y. Fang, M. Smith and M. Pepsin, “Effect of exogenous Methyl jasmonate in elicited anthocyanin producing cell cultures of Ohelo (Vaccinump ahalae)” , In Vitro Cell.Dev.Biol, pp. 106-113, 1998.
3. M. Rivas-San Vicente and J. Plasencia, "Salicylic acid beyond defence: its role in plant growth and development", Journal of Experimental Botany, vol. 62, no. 10, pp. 3321-3338, 2011.
4. War and H. Sharma, "Effect of jasmonic acid and salicylic acid induced resistance in groundnut onHelicoverpa armigera", Physiological Entomology, vol. 39, no. 2, pp. 136-142, 2014.
5. N. Obinata, T. Yamakawa, M. Takamiya, N. Tanaka, K. Ishimaru and T. Kodama, "Effects of Salicylic Acid on the Production of Procyanidin and Anthocyanin in Cultured Grape Cells", Plant Biotechnology, vol. 20, no. 2, pp. 105-111, 2003.
6. N. Chaichana and S. deeranupattana, “Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona” , International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 2, No. 3, pp. 146-150, 2012.