Open Access Journal

ISSN : 2394-6849 (Online)

International Journal of Engineering Research in Electronics and Communication Engineering(IJERECE)

Monthly Journal for Electronics and Communication Engineering

Open Access Journal

International Journal of Science Engineering and Management (IJSEM)

Monthly Journal for Science Engineering and Management

ISSN : 2456-1304 (Online)

Toll-like receptors and calcium-activated chloride/potassium channels as immunomodulators of allergic airway inflammation and asthma: a public health research experience in State of Nebraska, USA

Author : Saumya Pandey 1

Date of Publication :7th September 2017

Abstract: Allergic airway inflammation and asthma have emerged as major public health problem in the Midwest, United States of America. Targeting Toll-like receptor (TLR) signaling and Calcium-activated Chloride (ClCa)/Potassium (KCa) Channels in unraveling the cellular/molecular mechanisms underlying genetic susceptibility to inflammatory diseases in specific human patient population subset(s) is emerging as an attractive immunotherapeutic pharmacological strategy in management/prevention of asthma. Objectives: My pilot study aimed to investigate the role of TLRs and ClCa/KCa ion channels in human bronchial epithelial cells (NHBEC, BEAS-2B from ATCC), and eosinophils-derived from asthma patients of North American ethnicity. Methods: Whole cell patch clamp electrophysiological recordings were conducted for ClCa and KCa currents in cultured cells (passages P3-P7) grown on cover-slips. RNA and Protein were extracted from NHBECs, BEAS-2B and eosinophils using Trizol and RIPA methods. Pipette and bath solutions for electrophysiology were subjected to stringent pH and osmolality checks prior

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