Author : Jayant Pawar 1
Date of Publication :22nd December 2017
Abstract: This study presents the fractional characterization of bacteriocin-like material (Inhibiting molecule showing properties of bacteriocin) produced by Bacteriocin-like material was found to be active against Bacillus cereus and Salmonella typhimurium.. The maximum antibacterial activity of Pseudomonas aeruginosa was recorded at 16th hour incubation in nutrient broth at 370C. The antibacterial activity was found to be extracellular since, cell-free supernatant showed inhibition against test organism. The inhibitor was found to have proteinaceous nature and same has been confirmed by inactivation of inhibitor after addition of proteolytic enzymes (trypsin and proteinase K) and 0.2N NaOH for alkaline hydrolysis. After prolonged refrigeration and incubation at high temperature (600C, 1200C and under autoclave) for 15 minutes, inhibitor was found to be active against Bacillus cereus and Salmonella typhimurium. Therefore, inhibitor produced by the Pseudomonas aeruginosa can be bacteriocinlikematerial and may find application in medicine and in minimally processed food stuffs
Reference :
-
- Agnieszka Bogut & Maria Kozioł-Montewka. Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Poland: further evidence for the changing epidemiology of MRSA, NEW MICROBIOLOGICA, 2008, 31, 229-234,
- A.G. Ponce, M.R. Moreira, C.E. del Valle, S.I. Roura. ―Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables‖, LWT 41, 2008, 432–441.
- Ayad, E., Verheul, A., Jing, C., Wouters, J., & Smit, G. Antimicrobial producing wild lactococci from artisanal and non-dairy origins. International Dairy Journal, 2002,12, 145–150.
- Anja Kunert* et al. Immune Evasion of the Human Pathogen Pseudomonas aeruginosa: Elongation Factor Tuf Is a Factor H and Plasminogen Binding Protein., Journal of Immunology, 2007, 179, 2979 -2988 .
- C. Walsh, Where will new antibiotics come from? Nat. Rev. Microbiol. 1 , 2003, 65–70.