Author : Sambavi T R 1
Date of Publication :20th June 2019
Abstract: The second generation Biofuel production process of Lignocellulosic ethanol conversion is gaining rapid interest owing to the accelerated depletion of fossil fuels. The work was performed with an aim to produce in-house cellulases and reduce the major cost incurred (around 40% of the total fuel production cost) towards commercial cellulase procurement. A novel mixture containing equal volumes of the peels of potato, Sapodilla, Kiwi and coir was subjected to compositional analysis by the standard NREL procedure. The mixture was pre-treated using 15% (v/v) ammonia which showed around 70% lignin removal. In-house Cellulases were produced by innovative fixed volume solid repeated fed-batch from the above stated mixture using Trichoderma atroviride ATCC 28043. The in-house cellulases were concentrated 15 folds using a Tangential Flow Filtration unit and showed a percentage saccharification of 45% and percentage yield saccharification of 94.6% in an optimized saccharification process, performed within a Continuous stirred-tank reactor. The percentage yield of saccharification was 4% lesser than commercial cellulases while the cost for production of in-house cellulases was 15 folds reduced than the market cost of commercially available cellulases
Reference :
-
- C. Henry Lim and Hwa Sung Shin “Fed-Batch Cultures: Principles and Applications of Semi-Batch Bioreactors” doi:10.1017/CBO9781139018777, Cambridge series in chemical engineering, Cambridge University Press.
- Swapnil Bhargava, S. Kevin Wenger and R. Mark Marten. “Pulsed Feeding during Fed-Batch Aspergillus oryzae Fermentation Leads to Improved Oxygen Mass Transfer”. Biotechnology Progress, vol. 19, no.
- pp. 1091-1094. Sep. 1991. 3. PKR Kumar and BK Lonsane. “Potential of Fed-batch culture in Solid State Fermentation for Production of Giberellic Acid”. https://doi.org/10.1007/BF01024560, Biotechnology Letters. vol. 9, pp.179-182. March 1987.
- Sun Tao, Liu Beihui & Li Zuohu. “Enhanced Cellulase Production in Fed-Batch Solid State Fermentation of Trichoderma viride SL-1”, http://dx.doi.org/10.1002/(SICI)1097- 4660(199708)69:43.0.CO;2-Q, Journal of Chemical Technology and Biotechnology. vol. 69, pp 429-432. Aug 1997.
- M. E. Bushell, M. Rowe, C. A. Avignone-Rossa, J. N. Wardell. “Cyclic Fed-Batch Culture for Production of Human Serum Albumin in Pichia pastoris”, https://doi.org/10.1002/bit.10616, Biotechnology and Bioengineering. Vol. 82, pp. 678 – 683, June 2002.
- Vidhya Rangaswamy. “Improved Production of Gibberellic Acid by Fusarium moniliforme”. 10.5923/j.microbiology.20120203.02. Journal of Microbiology Research. vol.2, pp. 51-55. 2012.
- Behera, Sudhanshu & Ray, Ramesh. “Solid State Fermentation for Production of Microbial Cellulases: Recent Advances and Improvement Strategies”, 10.1016/j.ijbiomac.2015.10.090. International Journal of Biological Macromolecules. Vol. 86. Nov 2015.
- M A Shata, Hoda. “Extraction of milk-clotting enzyme produced by solid state fermentation of Aspergillus oryzae” Polish journal of microbiology, vol. 54 pp. 241-7. Feb 2005.
- G. Arifuzzaman Khan, M Shamsul Alam. “Thermal Characterization of Chemically Treated Coconut Husk Fibre” Indian Journal of Fibre and Textile Research, Vol. 37, pp. 20- 26. March 2012.
- Feumba Dibanda Romelle , Ashwini Rani P and Ragu Sai Manohar. “Chemical Composition of Some Selected Fruit Peels”, European Journal of Food Science and Technology, vol. 4, pp. 12-21. Sep 2016.