Author : Bhaskar Musmade 1
Date of Publication :26th October 2021
Abstract: A precise, robust and accurate method was developed for estimation of Fluocinolone Acetonide, Miconazole Nitrate, Chlorocresol, Methyl paraben and Prolyl Paraben, from cream dosage form. The gradient was optimized on BDS Hypersil C18, 250 x 4.6 nm 5μm column, operated at 45°C and mobile phase A and B were selected as monobasic phosphate buffer pH 7.2 and Acetonitrile, pumped at 1.5 ml/min. flow rate. All the solutions were injected at 20 μl injection volume and monitored at 238 nm. The Methylparaben, Propylparaben, Chlorocresol, Fluocinolone acetonide and Miconazole were eluted at about 4, 9, 9.8, 11and 25 minutes respectively. The recoveries were found between 98.0 to 102.0 % for each analytes. The method was found linear from 50 to 150 % of sample concentration and linear regression curves, (r2) were more than 0.999 for all the analytes. The developed method was remains unchanged after deliberate variation in method parameters hence proved the method robustness. The developed method is time saving, cost effective hence can be used in pharmaceutical industries for simultaneous estimation of these compounds from cream and other dosage forms.
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