Open Access Journal

ISSN : 2456-1304 (Online)

International Journal of Engineering Research in Electronics and Communication Engineering(IJERECE)

Monthly Journal for Electronics and Communication Engineering

Open Access Journal

International Journal of Science Engineering and Management (IJSEM)

Monthly Journal for Science Engineering and Management
Call For Paper : Vol 11, Issue 03, March 2024

ISSN : 2456-1304 (Online)

Call for Paper

Vol 11, Issue 03, March 2024

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Hydrocarbon Biodegradation Efficiency by Four Indigenous Bacterial Strains Isolated From Contaminated Soils

Author : Lumeshwari Sahu 1 Ranjana Shrivastava 2

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Date of Publication :1st October 2022

Abstract: Nineteen hydrocarbon degrading microorganisms were isolated from Ten hydrocarbon contaminated sites and were identified on the basis of morphological, biochemical and molecular characteristics as Acenetobacter junii, pantoea dispersa, Bacillus spizizenii, and Pseudomonas aeruginosa. The study illumined high density of bacteria acclimatized for biodegradation of hydrocarbon in soil. The isolates were examined for other hydrocarbon degradation in media supplemented with Diesel, Benzene, Petrol and Cyclohexane at three different concentrations viz 5%, 10% and 15% incubated for 3 different time intervals 5, 10 and 15 days. The results indicated that all the isolates possessed potential to degrade the wide variety of hydrocarbons. The most efficient among them was Acenetobacter junii which degraded all tested hydrocarbon showing maximum growth at 5% concentration and 10 days incubation. It could be concluded that native flora of hydrocarbon contaminated site adapt to the environmental condition and could be implicated to remove hydrocarbons.

Reference :

    1. Asquith, E.A., Geary, P.M., Nolan, A.L., Evans, C.A. 2012. Comparative Bioremediation of Petroleum Hydrocarbon-Contaminated Soil by Biostimulation, Bioaugmentation and Surfactant Addition. J. Env. Sci. Eng., A1, 637–650.
    2. Bakeas, E., Karavalakis, G., Fontaras, G., Stournas, S., 2011.An experimental study on the impact of biodiesel origin on the regulated and PAH emissions from a Euro 4 light-duty vehicle. Fuel 90 (11), 3200–3208
    3. Das, N., and Chandran, P. 2011. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. Biotech. Res. Inter.: 1–13.
    4. Dipti R. Parikh, Devayani R. Tipre, Nisha S. Nayak and Shailesh R. Dave. 2018. Degradation of Discarded used Engine Oil by Pseudomonas aeruginosa DP-1 and Its Optimization. Int.J.Curr.Microbiol.App.Sci. 7(04): 2224- 2229.
    5. Gina Lova Sari YulinahTrihadiningrum2 ,Ni’matuzahroh, 2019,Isolation and Identification of Native Bacteria from Total Petroleum Hydrocarbon Polluted Soil in Wonocolo Public Oilfields, Indonesia. Journal of Ecological Engineering. Volume 20, Issue 8, September pages 60-64.
    6. Holt, J.G., Kriey, N.R., Sneath, P.H.A., Staley, J.T. 1994. Bergey’s Manual of: Determinative Bacteriology. 9th edition. Lippincott Williams and Wilkins: Baltimore, USA
    7. Haritash, A.K., Kaushik, C.P., 2009. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. J. Hazard. Matter 169 (1–3), 1–15.
    8. Kanaly, R.A., Harayama, S., 2000. Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by bacteria. J. Bacteriol. 182 (8), 2059–2067.
    9. Leahy, J. G. and Colwell, R. R. 1990.Microbial degradation of hydrocarbons in the environment.Microbiol.Reviews. 54: 305-315.
    10. Obire O and Nwaubeta O 2001 Biodegradation of refined petroleum hydrocarbons in Soil, Journal of Applied Science and Environ.Management. 5:1, 43-46.
    11. Oudot J 1994 Soil pollution by petroleum products and treatments, Analysis.22:2. [12] Plaza, C., Xing, B.S., Fernandez, J.M., Senesi, N., Polo, A., 2009. Binding of polycyclic aromatic hydrocarbons by humic acids formed during composting. Environ. Pollut. 157, 257– 263.
    12. P. Singh, B. N. Tiwary 2017, Optimization of conditions for polycyclic aromatic hydrocarbons (PAHs) degradation by Pseudomonas stutzeri P2 isolated from Chirimiri coal mines.Biocatalysis and Agricultural Biotechnology 10 (2017) 20–29.
    13. Prakash, A., Bisht, S., Singh, J., Teotia, P., Kela, R., Kumar, V. 2014.Biodegradation Potential of Petroleum Hydrocarbons by Bacteria and Mixed Bacterial Consortium Isolated from Contaminated Sites.Turkish J. Eng. Env. Sci., 38, 41–50.
    14. Prince RC Elmendor DL, Lute JR, Hsu CS, Haith CE, Senius JD, Dechert GJ, Dougals GS, Butler EL, 1994 17alpha(h),21beta as a conserved internal marker For estimating the biodegradation of crude oil, Environ. Science.Tec.28: 142-145.
    15. Rahman, K. S. M., Rahman, T., Lakshmana P. P. and Banat. I. M. 2002, Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. Journal of Basic Microbiology. 42(4): 284-291.
    16. R.Gopinathan, M.Prakash and 2R.Bharathirajan, 2012, An experimental study for crude oil biodegradation in contaminated soil. Int.J.Curr.Microbiol.App.Sci (2012) 1(1): 12-16.
    17. Santhini, Myla, Sajani and Usharani, (2009).Screening of Micrococcus Sp from Oil Contaminated Soil with Reference to Bioremediation, Botany Research International 2 (4): 248-252
    18. Sudhir K Shekhar*, Jai Godheja and D.R. Mod., 2015,Hydrocarbon Bioremediation Efficiency by five Indigenous Bacterial Strains isolated from Contaminated Soils. Int.J.Curr.Microbiol.App.Sci (2015) 4(3) 892-905.
    19. Ulrici, W. 2000.Contaminant soil areas, different countries and contaminant monitoring of contaminants.Environmental Process II.Soil Decontamination Biotechnology H. J. Rehm and G. Reed, (Eds). 11:5-42.
    20. Wikstrom, P., Wiklund, A., Andersson, A. C. and Forsman, M. 1996. DNA recovery and PCR quantification of catechol 2,3- dioxygenase genes from different soil types. J. Biotechnol.52:107–120
    21. Xu, Y., and Liu, M. 2010. Bioremediation of Crude Oil-Contaminated Soil: Comparison of Different Biostimulation and Bioaugmentation Treatments. J. Hazard. Mater., 183, 395–401.
    22. Zhao, H.P., Wu, Q.S., Wang, L., Zhao, X.T., Gao, H.W., 2009. Degradation of phenanthrene by bacterial strain isolated from soil in oil refinery fields in Shanghai, China. J. Hazard. Mater. 164 (2–3), 863–869.

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