Date of Publication :7th December 2023

Abstract: The biosynthesis of silver nanoparticles (AgNPs) has been widely studied in several lines of research. Herbal and plant extracts have been commonly used for biosynthesis in green nanotechnology due to their lower toxicity. Gymnanthemum extensum, known as Nan Chao Woei in Thailand, is one of the traditional plants that possess various biological activities. Therefore, this research aims to synthesize AgNPs using G. extensum leaf extract. Optimal conditions were determined using UV-Vis spectrometric analysis. Results demonstrated a proper concentration of the plant extract at 20 µg/L. A silver nitrate concentration of 10 mM, a reaction time of 24 hr, pH 11, and a temperature of 80°C were found to be optimal for the formation of AgNPs. A characteristic of localized surface plasmon resonance in AgNPs was observed at a maximum wavelength of around 398-421 nm. The synthesized AgNPs displayed a size distribution of approximately 62.85-112.1 nm. Additionally, the antibacterial activity of AgNPs showed a minimum inhibitory concentration against Escherichia coli and Staphylococcus aureus of 8.86 and 17.72 µg/mL, respectively. In conclusion, the AgNPs were successfully synthesized by using G. extensum leaf extract. It could also be a potential one-step method for further studies and applications, providing a simple, rapid synthesis and an eco-friendly system.

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